Role in program: Program director, PI, project 2, co-investigator, project 3

Research Interests: Biotransformation of xenobiotics, especially environmental chemicals. Investigation of factors that affect the biotransformation of xenobiotics to more toxic or less toxic metabolites. Role of first pass biotransformation in modulating bioavailability. Influence of xenobiotics on steroid metabolism.

Selected Recent Publications

Doi, A., Lou, Z., Holmes, E. Li, C-L.J. Venugopalan, C., James, M.O. and Kleinow, K.M. Effect of micelle fatty acid composition and 3,4,3’,4’-tetrachlorobiphenyl (TCB) exposure upon intestinal [14C]-TCB bioavailability and biotransformation in channel catfishin situ preparations. Toxicological Sciences. 55: 85-95, 2000

Kleinow, K.M. and James, M.O. Response of the teleost gastrointestinal tract to xenobiotics. In “Target Organ Toxicity in Marine and Freshwater Teleosts.", W. H. Benson and D.R. Schlenk, editors. Taylor and Francis, London, New York, 2001, pp 269-362

James, M.O. Polychlorinated biphenyls: metabolism and metabolites. Proceedings of a workshop on “Recent Advances in the Environmental Toxicology and Health Effects of PCB” , L.W. Robertson and L. Hansen, eds, University of Kentucky Press, Lexington, 2001, pp 35-46

James, M.O., Tong, Z, Rowland-Faux, L., Venugopal, C.S. and Kleinow, K.M. Intestinal bioavailability and biotransformation of 3-hydroxybenzo(a)pyrene in an isolated perfused preparation from channel catfish, Ictalurus punctatus. Drug Metabolism and Disposition, 29: 721-728, 2001.

Van den Hurk, P., Kubiczak, G.A., Lehmler, H.J. and James, M.O. Hydroxylated polychlorinated biphenyl as inhibitors of the sulfation and glucuronidation of 3-hydroxybenzo(a)pyrene. Environ. Health Perspect. 110, 343-348, 2002

Lou, Z., Johnson, J.V. and James, M.O. Intestinal and hepatic microsomal metabolism of testosterone and progesterone by a 3?-hydroxysteroid dehydrogenase to the 3?-hydroxy derivatives in the channel catfish, Ictalurus punctatus. J. Steroid Biochem. and Mol. Biol. 82, 413-424, 2002.

Sugihara, N. and James, M.O. Binding of 3-hydroxybenzo(a)pyrene to hemoglobin and albumin. J. Biochem. Molec. Toxicol. 17 (4): 239-247, 2003

James, M.O. Kleinow, K.M., Zhang, Y., Zheng, R., Wang, L.Q. and Rowland-Faux, L. Increased toxicity of benzo(a)pyrene-7,8-dihydrodiol in the presence of polychlorobiphenylols. Marine Environmental Research, 58 (2-5), 343-346, 2004.

Sacco, J. and James, M.O. Glucuronidation in the polar bear (Ursus maritimus). Marine Environmental Research, 58 (2-5), 475-480, 2004.

Wang, L.Q., Falany, C.N. and James, M.O. Triclosan as a substrate and inhibitor of 3’-phosphoadenosine-5’-phosphosulfate sulfotransferase and UDP-glucuronosyl transferase in human liver fractions. Drug Metabolism and Disposition, 32: 1162-1169, 2004.

James, M.O., Lou, Z., Rowland-Faux, L. and Celander, M.C. Properties and regional expression of a CYP3A-like protein in channel catfish intestine. Aquatic Toxicology, 72: 362-371, 2005.

Wang, L-Q. Lehmler, H-J. Robertson, L.W., Falany, C.N. and James, M.O. In vitro Inhibition of human hepatic and cDNA-expressed sulfotransferase activity with 3-hydroxybenzo[a]pyrene by polychlorobiphenylols. Environmental Health Perspective, 113: 680-687, 2005

Sacco, J.C. and James, M.O. Sulfonation of environmental chemicals and their metabolites in the polar bear, Ursus maritimus. Drug Metab. Disp. 33(9): 1341-1348, 2005

Wang, L.Q. and James, M.O. Sulfotransferase 2A1 forms estradiol-17-sulfate and celecoxib switches the dominant product from estradiol-3-sulfate to estradiol-17-sulfate. J. Steroid Biochemistry and Molecular Biology 96(5): 367-374, 2005

Wang, L.Q. Lehmler, H.J., Robertson, L.W. and James, M.O. Polychlorobiphenylols are selective inhibitors of human phenol sulfotransferase 1A1 with p-nitrophenol as substrate. Chemico-Biological Interactions, 159:235-246, 2006

Wang, L.Q. and James, M.O. Inhibition of sulfotransferases by xenobiotics. Current Drug Metabolism 7: 83-104, 2006

Stuchal, L. Kleinow, K.M., Stegeman, J.J. and James, M.O. Demethylation of the pesticide methoxychlor in liver and intestine from untreated, methoxychlor-treated and 3-methylcholanthrene-treated channel catfish (Ictalurus punctatus): Evidence for roles of CYP1 and 3A family isozymes. Drug Metab. Disp. 34: 932-938, 2006

Doi, A.M., Lou, Z., Holmes, E., Venugopal, C.S., Nyagode, B., James, M.O. and Kleinow, K.M. Intestinal bioavailability and biotransformation of 3,4,3’,4’-tetrachlorobiphenyl in in situ preparations of channel catfish following dietary induction of CYP1A. Aquatic Toxicology 77: 33-42, 2006

Wang, L.Q. and James, M.O. Sulfonation of 17?-estradiol and inhibition of sulfotransferase activity by polychlorobiphenylols and celecoxib in Channel catfish, Ictalurus punctatus. Aquatic Toxicology, 81: 286-292, 2007